Thesis
Light sheet fluorescence microscopy for optical detection of magnetic resonance using the nitrogen-vacancy defect in nanodiamonds within whole organisms
- Creator
- Rights statement
- Awarding institution
- University of Strathclyde
- Date of award
- 2024
- Thesis identifier
- T17104
- Person Identifier (Local)
- 201864513
- Qualification Level
- Qualification Name
- Department, School or Faculty
- Abstract
- The research motivation for this thesis was the design, construction, and operation of a Light-sheet Fluorescent Microscope (LSFM) that is capable of imaging freely moving whole organisms, performing Optically Detected Magnetic Resonance (ODMR) measurements, and using Adaptive Optics (AO) elements. The separation of illumination and detection paths in a LSFM allows it to be a highly adaptable microscope. A variety of LSFM designs have been presented in the literature, each designed to fulfill the specific imaging and sample requirements. This work presents an upright dual illumination single detection LSFM design. Two lightsheets are generated via cylindrical lenses, manipulated through a single Spatial Light Modulator (SLM), and are capable of illuminating a large volume, capturing freely moving Caenorhabditis elegans, or C. elegans at a cellular resolution. C. elegans was chosen as an imaging sample due to its small size, optical transparency, and well known biology. Therefore, this work emphasised on the characterisation of the LSFM microscope rather than answering a biological question. Fluorescent Nanodiamonds (FND) with a Nitrogen-Vacancy (NV) defect imaging is presented in this work. FNDs are biocompatible and offer an exceptional stability against photo-generated damage. FNDs imaging paves the way for the investigation of cell and whole organism tracking and identification leading to the examination of further biological processes. Additionally, the NV spin levels can be optically detected, when microwaves are applied, as spin transitions are observed as dips in fluorescence, leading to ODMR measurements. NV properties in combination with ODMR lead to temperature, magnetic field, and electric sensing. This work demonstrates ODMR measurements using the detection path of the LSFM with wide-field illumination and LS illumination. ODMR measurements are recorded when a Radio Frequency (RF) signal is applied to a NV centre and control its spin state. The effect of the RF signal power was examined showing a direct correlation of the RF signal power and the ODMR spectrum measurement performance. The effect of a magnetic field was also examined, showing magnetometry capabilities of the LSFM ODMR configuration.
- Advisor / supervisor
- Patton, Brian, 1978-
- Resource Type
- DOI
关系
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PDF of thesis T17104 | 2024-12-10 | 公开 | 下载 |