Thesis

Development of a novel monoclonal antibody-based immunoaffinity column for the detction of aflatoxins

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Awarding institution
  • University of Strathclyde
Date of award
  • 2010
Thesis identifier
  • T12567
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Abstract
  • 1G7-1E2 is a novel monoclonal antibody, created at the University of Strathclyde in Glasgow. Several IG7-1E2 antibodies were produced by poly ethylene gycol fusion of mouse myeloma cells with sensitised spleen cells isolated from Balb c/NZB F1 hybrid mice immunised intraperitoneally with aflatoxin B1 and ochratoxin A protein conjugates. The main characteristic of these monoclonal antibodies is their ability to bind sufficiently better to all four main aflatoxins and ochratoxin A than other antibodies already on the market. Past studies have shown how useful these antibodies can be when used as diagnostic tools, such as in an enzyme linked immunoabsorbent assay, (Candlish, 1984, Prasertsilpa, 1999), however, they have not been tried and tested in other applications to date. -- In this study, a set of immunoaffinity columns were produced in order to achieve one main aim; to produce a diagnostic tool that has the ability to compete with others already established on the immunodiagnostic market, whilst making use of 1G7-1E2. The results provide evidence that 1G7-1E2 has the ability to meet this aim. The immunoaffinity columns produced capture a higher amount of aflatoxin B1, G1 and G2 than current columns on the market. As an additional experiment, a set of enzyme linked immunoabsorbent assays were produced in an attempt to discover the component responsible for the antibody’s affinity towards other mycotoxins found in foodstuffs and feed. The outcome of this is still undetermined.
Resource Type
DOI
Date Created
  • 2010
Former identifier
  • 814339

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