Thesis
Potential wound healing properties of marine organisms
- Creator
- Rights statement
- Awarding institution
- University of Strathclyde
- Date of award
- 2012
- Thesis identifier
- T13468
- Qualification Level
- Qualification Name
- Department, School or Faculty
- Abstract
- Naturally derived marine compounds have been used in wound healing for thousands of years. However, we have only scratched the surface with respect to discovering the potential that compounds from the ocean can have to improve this area. The overall aim of this study was to screen a variety of compounds, provided by GlycoMar Ltd, derived from marine organisms, to determine if they had any influence on the wound healing process. Samples were assessed for their proliferative, cytotoxic and antimicrobial properties to macrophage like cells (THP-1) representing one of the cell types that would be found in the wound healing response. Algae samples 2211002 and 03021003 and MIX025 (fucoidan) increased THP-1 cell proliferation compared with the FCS control while algae samples 15091001, 18041104, 15091002 and ID539 (fucoidan), C4384 (chondroitin sulphate) and H3393 (heparin) inhibited proliferation. Cytotoxicity of ID539, C4384 and H3393 was assessed by an Alamarblue metabolic assay to confirm cell inhibition was occurring and not cell death. Algae samples were tested for antimicrobial activity using a disc diffusion assay on agar plates and found to be antimicrobial at 1mg/ml against E. coli, S. aureus and B. subtilis but not P. aeruginosa. From this algae samples 02081002, 09071003, 03021003 were tested for their antimicrobial activity against E. coli using an Alamarblue assay and these samples were deemed antimicrobial at concentrations of 500ng/ml and higher. 5 Following this work, samples were tested using the immortalised epithelial cell line MCF-7 as a model of the epithelium to look for wound healing properties such as cell adhesion and cell migration. Using crystal violet staining to measure cell adhesion, only sample 06081003 significantly increased cell adhesion in comparison with the control. Using Ibidi inserts to create an artificial gap during cell growth cell migration into the site was measured and samples 03032003 (algae), 09071007 (algae), ID536 (fucoidan), MIX025 (fucoidan) all significantly (P<0.05) increased cell migration at both 1mg/ml and 0.1mg/ml. This study was to undertake a broad analysis of a variety of marine derived compounds for potential use in wound healing. The results show that many of these samples have properties, which could be beneficial in a wound healing capacity. Further study to continue to understand the mechanisms of action of those samples which have shown to have properties which fit the criteria for wound healing compounds would allow these to be perhaps in the future be developed into commercial products.
- Resource Type
- DOI
- Date Created
- 2012
- Former identifier
- 996250
关系
项目
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File | 2021-07-02 | University of Strathclyde |