Thesis
Integrated bioprocessing for the sustainable manufacture of poly (methyl methacrylate) precursors
- Creator
- Rights statement
- Awarding institution
- University of Strathclyde
- Date of award
- 2019
- Thesis identifier
- T15262
- Person Identifier (Local)
- 201474386
- Qualification Level
- Qualification Name
- Department, School or Faculty
- Abstract
- Industrial focus is shifting from the utilisation of petrochemicals and is moving towards the utilisation of biomass for the production of platform chemicals. The finite lifespan of petrochemicals, the dramatic fluctuation in the price of oil and environmental concerns are all significant drivers for this change. Polymers such as poly (methyl methacrylate), a mass market polymer, can be sustainably produced using platform chemicals derived from biomass. This study investigates the production of two platform chemicals, mesaconate and butyl-methacrylate, from biomass which would allow the sustainable production of poly (methyl methacrylate). Mesaconate was initially targeted with butyl methacrylate later identified as a potential target with envisaged reduced downstream processing required for integration onto poly (methyl methacrylate) production. Initially a mesaconate production pathway was incorporated into Corynebacterium glutamicum selected due to the inherent ability of C. glutamicum to produce large quantities of glutamate. Mesaconate production was demonstrated through bioprocesses containing the constructed Escherichia coli strains with improvement in the analysis method providing accurate quantification. Adaption of the bioprocess in particular through alteration of the dissolved oxygen improved the mesaconate titre further. Secondly a novel pathway for the production of a poly (methyl methacrylate) precursor butyl methacrylate was constructed within E. coli. Strains containing this pathway demonstrated production of butyl methacrylate within biotransformations. Adaptation of the biotransformation method and strain development produced an increase in the butyl methacrylate titre. Further engineering allowed the elimination of by-products from the bioprocess and improved the tolerance of the host to the toxicity of butyl methacrylate. Production of butyl methacrylate directly from glucose was attempted although this was not successful.
- Advisor / supervisor
- Arnold, Alison
- Harvey, Linda
- McNeil, Brian, 1954-
- Resource Type
- Note
- This thesis was previously held under moratorium from 23/08/2019 to 23/08/2024
- DOI
- Date Created
- 2019
- Former identifier
- 9912708793202996
Relazioni
Articoli
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PDF of thesis T15262 | 2021-07-02 | Pubblico | Scaricare |