Thesis

Characterization of chalcone synthase genes in red raspberry cultivar (rubus idaeus)

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Awarding institution
  • University of Strathclyde
Date of award
  • 2014
Thesis identifier
  • T13945
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Abstract
  • The biosynthesis of anthocyanin compounds involves type III polyketide synthases (PKSs): chalcone synthase (CHS), which is the first enzyme of the flavonoid synthetic pathways leading to synthesis of various anthocyanins. The European red raspberries are a commercially important fruit that needed both for fresh fruit consuming and food manufacturing. The fruits are a valuable source of nutraceuticals, notably anti-oxidants by anthocyanins that are pigments conferring impressive red colour to the fruits. To improve the quality in red raspberry through increasing anthocyanin content, the main objective was focused on investigation of the PKS genes that are an essential for the biosynthesis of anthocyanins. The aim of this thesis was to characterize the PKS/CHS gene regions within the three Bacterial Artificial Chromosome clones (BACs), which originated from the genome of the European red raspberries (Rubus idaeus) cv. Glen Moy (SCRI), using 454 sequencing technology assisted by a systematic construction of fosmid libraries. The two BACs (29M05 and 24B12) always presented similarities in almost analyses resulting from the presence of a 53 kb overlap that was revealed by assemblies of resulting 454 sequencing as it may be possible that there is no PKS/CHS gene in another one, BAC31B12. The assembly of BAC24P12 was completed, with an entire DNA length of 117 kb but BAC29M05 could not be completed along all its length of ~170 kb as the longest contig was 96 Kb provided the most important information of this BAC whilst containing a complete PKS1 gene sequence, currently identified as Naringenin chalcone-PKS. The alignments of assembled sequences also strengthened the similarities between these two BACs both at nucleotide and amino acid sequences. All four PKS/CHS gene regions found in the both BACs presented being multiple PKS/CHS gene family with conservation of an intron and two exons of gene structure as variations of those sequence confirmed being PKS/CHS pseudogenes in plant genome. The two informative sources generated in this thesis, including the fosmid sub-libraries, which are availably manageable sources for validating the BAC sequences, and physical map drafts provided better information of this chromosomal segment in this thesis, as they will be useful available tools for further studying in red raspberry genome.
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DOI
Date Created
  • 2014
Former identifier
  • 1088946

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