Thesis

Inter-strain variation of pseudomonas aeruginosa : studies on virulence and variation of sigma 54 dependent regulatory targets

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Awarding institution
  • University of Strathclyde
Date of award
  • 2012
Thesis identifier
  • T13204
Qualification Level
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Department, School or Faculty
Abstract
  • Pseudomonas aeruginosa is a widespread Gram-negative, facultative anaerobic bacterium that is usually motile via one or more flagella. Its ability to infect a wide range of organisms is due to the possession of diverse colonisation and virulence-related genes. Transcriptional regulation of these genes is mediated by alternate sigma factors, regulatory proteins such as enhancer binding proteins (EBPs) and anti-sigma factors. We have sequenced twelve P. aeruginosa strains and used comparative genomics to investigate genes that are regulated by sigma 54 and EBPs, which are involved in attachment, biofilm formation and toxin secretion. We have analysed differences in these systems at the genomic level and coupled them to their phenotypic consequence in virulence between various clinical isolates including isogenic pairs as well as lab evolved and environmental and isolates. This analysis has revealed remarkable plasticity in the complement of sigma 54 target genes within the P. aeruginosa lineage.
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Note
  • Strathclyde theses - ask staff. Thesis no. : T13204
DOI
Date Created
  • 2012
Former identifier
  • 947833

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