Thesis
Non-invasive impedance monitoring of vascular cell growth on coronary stents
- Creator
- Rights statement
- Awarding institution
- University of Strathclyde
- Date of award
- 2014
- Thesis identifier
- T13951
- Qualification Level
- Qualification Name
- Department, School or Faculty
- Abstract
- Coronary heart disease is the leading cause of death in developed countries. Implantation of a stent, by percutaneous coronary intervention, has emerged as a safe and widely used procedure to address this problem. However, a number of limitations remain with this technique, including potentially delayed healing of the artery endothelium. Recovery of the endothelium is a crucial factor in stent performance and has a strong influence on long term outcomes for the patient. Despite this, the precise mechanisms controlling re-endothelialisation are unclear and there are no means of non-invasively monitoring this process in vivo.Existing real time monitoring of impedance in cell cultures provides information related to cell activity, adherence and confluence. Therefore, collection of such information applied to coronary stents may be a first step towards a better understanding of their re-endothelialisation. This may also help in understanding the exact duration of antiplatelet therapy required and thus lower treatment costs and improve the long term patient outcomes.This project investigated the suitability of impedance spectroscopy as a real time technique for monitoring endothelial adhesion and growth on metal surfaces. Primary endothelial cells isolated from freshly excised porcine pulmonary artery were cultured successfully in vitro on a self-manufactured bio-sensor. The cells were also seeded onto a commercially available coronary stent and impedance measurements were made over time. Impedance measurements were performed at different stages of the cell growth in vitro.It was found that there was a relatively high degree of variability in the impedance measurements obtained. Further analysis revealed that this variability was related to variations in the manufacture process of the biosensors. In a small number of comparable experiments with low variability (n=3), the impedance values collected from the biosensor devices were found to be correlated with cell number. These encouraging findings warrant further investigation of the potential of impedance measurements for monitoring re-endothelialisation of coronary stents in real time.
- Resource Type
- Note
- This thesis was previously held under moratorium from 24th March 2015 until 24th March 2017.
- DOI
- Date Created
- 2014
- Former identifier
- 9912173063402996
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