Thesis

Application of electrochemical based sensors in support of toxicokinetic and pharmacokinetics

Creator

Mahammed, Farjana

Rights statement

Strathclyde Thesis Copyright

Awarding institution

University of Strathclyde

Date of award

2020

Thesis identifier

T16426

Person Identifier (Local)

201777369

Qualification Level

Masters (Postgraduate)

Qualification Name

Master of Philosophy (MPhil)

Department, School or Faculty

Department of Pure and Applied Chemistry

Abstract

Presently, the capability of electrochemical aptamer-based sensors (E-AB) are being explored, which incorporate aptamers for sensing small molecules and proteins, transducing the signal electrochemically. Currently, much work around E-ABs is being conducted by a group at the University of California, Santa Barbara for recognising aminoglycoside antibiotics, inclusive of tobramycin. To validate the accuracy of the biosensor measurements, a proof of concept is required to compare E-AB results with traditional samples. For this there is a need for a simple, reliable and sensitive method for quantification of tobramycin. Two techniques were explored, resulting in different endpoints of a quick qualitative measurement, compared to a more quantitative result. Firstly, a liquid chromatography tandem mass spectrometry (LC-MS/MS) assay has been successfully developed for the determination of tobramycin in rat plasma, over a linear dynamic range of 50 - 10,000 ng/mL, using protein precipitation (PPT) and multiple reaction monitoring (MRM). Secondly, as an alternative method of detection, electrochemiluminescence (ECL) was investigated to detect tobramycin using screen printed electrodes (SPE) modified with a nafion-ruthenium film, down to 2.34 µM. Overall, ECL showed promise as a detection method and as a technique for screening, providing a qualitative assessment for the presence of tobramycin. Further work is required, however in the future LC-MS/MS could be utilised to provide a quantitative result for tobramycin in rat plasma, after initial ECL measurements. These techniques could be used in the future to support output of biosensor measurements.

Advisor / supervisor

Dennany, Lynn

Resource Type

Master thesis

Note

Previously held under moratorium from 17th June 2020 until 22nd November 2022. GSK - held in Chemistry Department during moratorium.

DOI

10.48730/fsfz-fq31

Funder

GlaxoSmithKline

Embargo Note

This thesis is restricted to Strathclyde users only until 17th June 2025

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