Thesis

Application of electrochemical based sensors in support of toxicokinetic and pharmacokinetics

Creator
Rights statement
Awarding institution
  • University of Strathclyde
Date of award
  • 2020
Thesis identifier
  • T16426
Person Identifier (Local)
  • 201777369
Qualification Level
Qualification Name
Department, School or Faculty
Abstract
  • Presently, the capability of electrochemical aptamer-based sensors (E-AB) are being explored, which incorporate aptamers for sensing small molecules and proteins, transducing the signal electrochemically. Currently, much work around E-ABs is being conducted by a group at the University of California, Santa Barbara for recognising aminoglycoside antibiotics, inclusive of tobramycin. To validate the accuracy of the biosensor measurements, a proof of concept is required to compare E-AB results with traditional samples. For this there is a need for a simple, reliable and sensitive method for quantification of tobramycin. Two techniques were explored, resulting in different endpoints of a quick qualitative measurement, compared to a more quantitative result.Firstly, a liquid chromatography tandem mass spectrometry (LC-MS/MS) assay has beensuccessfully developed for the determination of tobramycin in rat plasma, over a linear dynamic range of 50 - 10,000 ng/mL, using protein precipitation (PPT) and multiple reaction monitoring (MRM). Secondly, as an alternative method of detection, electrochemiluminescence (ECL) was investigated to detect tobramycin using screen printed electrodes (SPE) modified with a nafion-ruthenium film, down to 2.34 µM. Overall, ECL showed promise as a detection method and as a technique for screening, providing a qualitative assessment for the presence of tobramycin. Further work is required, however in the future LC-MS/MS could be utilised to provide a quantitative result for tobramycin in rat plasma, after initial ECL measurements. These techniques could be used in the future to support output of biosensor measurements.
Advisor / supervisor
  • Dennany, Lynn
Resource Type
Note
  • Previously held under moratorium from 17th June 2020 until 22nd November 2022. GSK - held in Chemistry Department during moratorium.
DOI
Funder
Embargo Note
  • This thesis is restricted to Strathclyde users only until 17th June 2025

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