Thesis

Inactivation of microorganisms isolated from infected lower limb arthroplasty using High-Intensity Narrow-Spectrum (HINS) light

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Awarding institution
  • University of Strathclyde
Date of award
  • 2011
Thesis identifier
  • T13141
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Abstract
  • Infection rates following arthroplasty surgery are reported between 1-2%, with considerably higher rates in revision surgery. The associated costs of treating infected arthroplasty cases are over four times the cost of the primary procedures, with significantly worse functional and satisfaction outcomes. In addition, multiple antibiotic resistant bacteria are developing, so to reduce the infection rates and costs associated with arthroplasty surgery, new preventative methods are required. HINS-light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and is proven to have bactericidal activity against a wide range of species. The aim of this study was to investigate the efficacy of HINS-light for the inactivation of microorganisms isolated from infected arthroplasty cases. Specimens from hip and knee arthroplasty infections are routinely collected in order to identify possible causative organisms and susceptibility patterns. This study tested a range of these isolates for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also set-up. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log reduction) was achieved for all of the clinical isolates from infected arthroplasty cases. The typical inactivation curve showed a slow initial reaction followed by a period of rapid inactivation. The doses of HINS-light exposure required ranged from 118 - 2214 J/cm2 respectively. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. A similar trend was also found when isolates were exposed whilst seeded on agar surfaces. As HINS-light utilises visible-light wavelengths it can be safely used in the presence of patients and staff. This unique feature could lead to possible applications such as use as an infection prevention tool during surgery and post-operative dressing changes.
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  • Strathclyde theses - ask staff. Thesis no. : T13141
DOI
Date Created
  • 2011
Former identifier
  • 946791

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