Thesis

Design and evaluation of bacteriophage production for potential commercial use

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Awarding institution
  • University of Strathclyde
Date of award
  • 2011
Thesis identifier
  • T12876
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Department, School or Faculty
Abstract
  • The purpose of this research was to evaluate the production of bacteriophages in a commercial plant. Bacteriophage K, a broad host range bacteriophage which is active against a wide range of staphylococci including MRSA, was used as a typical bacteriophage. There has been increased interest in the potential use of bacteriophage in pharmaceutical, agricultural and other areas. There are several techniques used for bacteriophage growth on a small-scale, however, large scale production has not been examined. In these studies, the production of bacteriophage K in S. aureus 8588 has been investigated. Medium optimization, the bacterial host growth conditions and infection and lysis conditions were investigated. The optimal condition for the growth of bacterial host were in a medium containing amino acids as carbon sources, in aerobic condition, at 37°C; these conditions were also the optimal conditions for infection and lysis. By feeding continuously bacterial host cells in their stationary phase into a bacteriophage lysate, bacteriophages were produced at all dilution rates (0.5-2.61 hr-1) and remained in a steady state. Moreover, there was no wash out observed, even at the time exceeding the average bacteriophage growth period. In addition, immobilization of the bacteriophage on nylon membrane by corona discharge was examined for use as a potential wound dressing. The result showed that the bacteriophages were immobilized on the nylon strip and remained active.
Resource Type
DOI
Date Created
  • 2011
Former identifier
  • 833008

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