Thesis

Studies of anti-trypanosomal active triterpenoids from some African propolis

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Awarding institution
  • University of Strathclyde
Date of award
  • 2012
Thesis identifier
  • T13333
Qualification Level
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Department, School or Faculty
Abstract
  • There is a drastic need for new and improved drugs for parasitic diseases such as African Trypanosomiasis which is a major global health problem with high endemicity in developing countries due to the alarming rate of resurgence of the disease, drug related adverse effects and drawbacks of the existing useful drugs. Natural products have continued to play a significant role to the search for new, affordable and potent anti-trypanosomal drugs with minimal side effects. Bees have the natural way of concentrating higher yields of secondary metabolites in propolis samples when compared to that of the plant source. The aim of this study was to evaluate the fraction VLC6 of the propolis sample and the isolated triterpenes for their anti-trypanosomal activity using an Alamar Blue assay. The chromatographic separation of the fraction VLC6 of propolis sample from Cameroon resulted in the isolation of the fraction 710.58Fr8 with a relatively high yield of (26.1 mg) which exhibited 1.7 % of the negative control values when tested against the bloodstream form of Trypanosoma brucei brucei S427 at a single concentration of 20 μg/ml. By means of analysis using thin layer chromatography and nuclear magnetic resonance spectroscopy (NMR) one triterpene compound was identified from 710.58Fr8 and its structure was elucidated as Ambolic acid.
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Note
  • Strathclyde theses - ask staff. Thesis no. : T13333
DOI
Date Created
  • 2012
Former identifier
  • 967042

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