Thesis

Developing electrochemical methods for monitoring of therapeutic drugs

Creator
Rights statement
Awarding institution
  • University of Strathclyde
Date of award
  • 2024
Thesis identifier
  • T17164
Person Identifier (Local)
  • 202175758
Qualification Level
Qualification Name
Department, School or Faculty
Abstract
  • The ability to move towards personalized medicine has been hampered by challenges including the point-of-care monitoring of pharmaceutical drugs and their metabolites. To allow clinicians the ability to adapt treatment regimens requires this detail. Therefore, the ability to identify pharmaceutical drugs and their metabolites as well as biomarkers is vital to process this area of research. Current methods are inadequate for this point-of-care analysis and often require extraction or pre-concentration of the biological samples. As such, it has become necessary to develop a robust detection methodology to monitor these targets in complex biological matrices directly within these matrices and with rapid results. To address this challenge, this study examined the use of electroanalytical tools, in particular, electrochemiluminescence (ECL) and square wave voltammetry (SWV). The developed SWV sensor was successfully applied for the detection of leucovorin (LV) and its metabolite, 5-methyltetrahydrofolic acid (5-MTHF) at clinically relevant concentrations in artificial salvia, urine and human pooled serum. Point-of-care use was the primary focus of this study and so the developed sensor would need to be used by non-experts and at the point-of-use. Therefore, all analysis was performed without extraction, purification or separation strategies. SWV was shown to be effective at simultaneously detecting both LV and 5-MTHF in artificial urine. Increased sensitivity was achieved by a simple dilution of the matrix, facilitating a lower limit of detection to be attained.
Advisor / supervisor
  • Dennany, Lynn
Resource Type
DOI
Embargo Note
  • The digital version of this thesis is available to Strathclyde users only until 12/12/2029

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