Thesis
Miniaturization of light-sheet microscopy systems using MEMS as active optical elements
- Creator
- Rights statement
- Awarding institution
- University of Strathclyde
- Date of award
- 2024
- Thesis identifier
- T17168
- Person Identifier (Local)
- 201784848
- Qualification Level
- Qualification Name
- Department, School or Faculty
- Abstract
- Light-sheet microscopy is widely recognised in the field of bioimaging as one of themain options when it comes to 3D imaging of large cleared samples or live imagingof organisms. This can be mainly attributed to certain advantages that result from itsunique orthogonal optical geometry, such as low photobleaching and fast acquisitionof 3D volumes. This work introduces two compact light-sheet microscopy systemsthat explore the miniaturization of the technique, with the use of small-scale devicesthat control the operation of the illumination and imaging path. In the two mainsystems presented in this work two different 2D micro-electromechanical systems(MEMS) mirrors are used for the generation of the scanned light-sheet in one axis, aswell as, for the parallel translation of the light-sheet on the orthogonal axis. In this waythe parallel planes of the sample can be illuminated without the need of a mechanicalstage. This type of optical translation is coupled with the use of a tunable lens in theimaging path that refocuses to each subsequent illuminated plane. The result of this isa stage-free microscope with all optical scanning. An exploration to the use of asecondary tunable lens is also presented in this work as a tool for homogenising thelight-sheet thickness throughout the field of view (FOV) by a post processing methodof image “tiling”. Both the MEMS and the tunable lens are small scale devices that notonly contribute in a microscope with compact footprint but can equally reduce theoverall cost of the device with relatively inexpensive pricing. In the same spirit thedevice is built with custom 3D printed holders and off-the-shelf optomechanical parts.The use of the 3D printer has also been investigated in the field of optical componentdesign, with a custom-made 3D printed prism that was developed to correct aberrationsand allow imaging of microscope slides at an angle. Different test samples are used tocharacterize the optical paths of the systems as well as its overall imaging performance.The collection of z stacks is accomplished with the use of a custom control softwarewhereas attention is given to different ways of visualizing the 3D stacks that areacquired with the microscope.
- Advisor / supervisor
- Uttamchandani, Deepak
- Bauer, Ralf
- Resource Type
- DOI
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